11/20/2022 0 Comments Sononym for classification![]() ![]() In addition, the G+C content of DNA was extended up to 37–55 mol% ( Park et al., 2016). Subsequently, the genus was emended due to the newly obtained data, including the presence of polar, bipolar, or lateral flagella, gelatin and Tween 80 hydrolysis, glucose fermentation, and the ability of some strains to produce buds and prosthecae ( Ivanova et al., 2002a Hwang et al., 2016 Beurmann et al., 2017). All species of the genus were oxidase-positive, required a seawater base for growth, and did not accumulate poly-p-hydroxybutyrate. ![]() Cells of the genus were described as Gram-stain-negative, aerobic, chemoorganotrophic, non-spore-forming, straight and curved rods or ovoid, those are motile by means of a single polar flagellum. At the time of writing, the genus Pseudoalteromonas comprises 49 validly published species, including Pseudoalteromonas haloplanktis as the type species. (1995) by splitting the genus Alteromonas into two genera due to its high level of heterogeneity. The genus Pseudoalteromonas was proposed by Gauthier et al. distincta based on the rules of priority with the emendation of the species. Also, we have proposed to reclassify Pseudoalteromonas paragorgicola as a later heterotypic synonym of P. According to phylogenetic evidence and similarity for the chemotaxonomic and genotypic properties, the strain 16-SW-7 (= KCTC 52772 = KMM 701) represents a novel member of the species Pseudoalteromonas distincta. The strain 16-SW-7 has the ANI values of 98.2% with KMM 638 T and KMM 3548 T and the DDH values of 84.4 and 83.5%, respectively, indicating clearly that the three strains belonged to a single species. The finished genome of the strain 16-SW-7 (GenBank assembly accession number: GCA_005877035.1) has a size of 4,531,445 bp and comprises two circular chromosomes L1 and S1, deposited in the GenBank under the accession numbers CP040558 and CP040559, respectively. ![]() The polar lipid profile was characterized by the presence of phosphatidylethanolamine, phosphatidylglycerol, two unidentified amino lipids, and three unidentified lipids. The genomic DNA G+C content was 39.3 mol%. ![]() The strain 16-SW-7 grew with 1–10% NaCl and at 4–34☌, and hydrolyzed casein, gelatin, tyrosine, and DNA. The closest relatives of the environmental isolate were Pseudoalteromonas distincta KMM 638 T and Pseudoalteromonas paragorgicola KMM 3548 T, with the plural paralogous 16S rRNA genes of 99.87–100% similarity. The phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain 16-SW-7 is a member of the Gammaproteobacteria genus Pseudoalteromonas. 4Department of Bioeconomy and Food Security, School of Economics and Management, Far Eastern Federal University, Vladivostok, RussiaĪ strictly aerobic, Gram-stain-negative, rod-shaped, and motile bacterium, designated strain 16-SW-7, isolated from a seawater sample, was investigated in detail due to its ability to produce a unique α-galactosidase converting B red blood cells into the universal type blood cells.Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia 2Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia Nedashkovkaya 1*, Song-Gun Kim 2, Larissa A. ![]()
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